For the diagnosis of tetanus, it mainly relies on relevant medical history and clinical manifestations. In terms of laboratory diagnosis, most cases involve culture of Clostridium tetani from wound tissue or polymerase chain reaction testing. Generally, a positive result can confirm a diagnosis of tetanus, but a negative result does not rule it out. Most laboratories are unable to perform these tests, and even if tetanus antibodies reach a protective level, it does not exclude a diagnosis of tetanus. If the patient has a clear history of injury or animal bites, and presents with symptoms such as lockjaw, a sardonic smile, muscle rigidity, difficulty swallowing, or persistent spasms, tetanus should be considered promptly and relevant treatment initiated.