Ventricular septal defect generally is not hereditary, and its genetic DNA should not be problematic. Therefore, the amniocentesis for patients with ventricular septal defect could potentially be problem-free. Although amniocentesis is not very diagnostic, it is still recommended for older patients or those with high risks of malformations or genetic variations. If there is only concern about a ventricular septal defect, a four-dimensional color Doppler ultrasound can be performed. Particularly after the 22nd week of pregnancy, a four-dimensional cardiac ultrasound can help in identification. However, some very small defects might not be detectable. But typically, a minor, isolated ventricular septal defect might impact heart function but generally does not significantly affect the growth and development of the child.