If the body exhibits symptoms of coughing, and persistent cough which highly suspects whooping cough, bacterial culture can be performed. Initially, a nasopharyngeal swab can be taken; during the spasmodic phase of coughing, sputum culture can be chosen for specimen collection for bacterial culture, with a higher positive rate in the early stages. Fluorescent antibody staining can also be conducted, which involves making a smear from a nasopharyngeal swab, aiding in rapid diagnosis. Serological tests can also be performed, conducting double serum agglutination tests and complement fixation tests, and if the antibody titer rises, it can be confirmed as an infection of this disease.