The definitive diagnosis of male infertility requires determination based on professional examination procedures. Common examinations include the following: First, it is crucial to conduct a medical history interview. The patient should actively cooperate and honestly report their conditions, such as whether they have had mumps, any unsafe sexual behaviors, and what their occupation is. Second, a physical examination, including a general body and reproductive organ examination, is necessary. Special attention should be given to the patient's developmental, nutritional, and mental status, with a focus on the development of the reproductive organs. Third, laboratory tests. The most common is semen analysis. Other tests may be selected based on specific conditions, such as X-ray examinations to determine the location of any blockage in the vas deferens. Techniques such as vasography, epididymography, vas deferens and seminal vesicle radiography, or urethrography can be used. Testicular exams are also performed to assess the condition of the testicles. Additionally, immunological tests, including sperm agglutination and immobilization tests, are used to detect sperm agglutination antibodies or immobilization antibodies in serum or seminal plasma, which help assess the immune status.