The main cause of premature rupture of membranes leading to preterm birth is infection of the reproductive tract, including vaginitis and cervicitis. These inflammations can ascend along the vagina and uterus, affecting the amniotic membrane and leading to its damage, which causes premature rupture of the membrane. Therefore, women should receive timely treatment for any gynecological infections before or during pregnancy, and should not take them lightly. Additionally, high pressure in the amniotic cavity, such as with multiple pregnancies or excessive amniotic fluid, can exceed the tolerance of the amniotic membrane and cause it to rupture prematurely. Moreover, physical trauma to the mother, sexual intercourse during pregnancy with excessive movement, or improper amniocentesis can also lead to premature rupture of the membranes.